

The remaining position battles are shown. The practices and performances of several teams are also aired in this episode. The first-place trainee overall in each category will receive a 100,000 vote bonus. The first-place trainee in each group will receive a bonus of 10,000 votes, with the exception of the hidden song, where the first place will gain a 20,000 vote bonus. There are 3 songs for vocals ( Ikimonogakari's "Sayonara Seishun", Shota Shimizu's "Hanataba no Kawari ni Melody wo", & Official Hige Dandism's "Pretender"), 3 songs for dance ( Seventeen's "Fallin' Flower", Nissy's "NA", JO1's "Oh-Eh-Oh"), 2 songs for rap ( Ken the 390's "Overall" and "Nobody Else"), and 1 hidden song (later revealed to be BTS' "Dynamite"). The trainees are tasked to perform live in groups, based in positions they choose: vocal, dance, or rap. The next evaluation is announced to be the position battles. Naoki Kose reveals that he has dropped out from the competition due to complications caused by COVID-19 resulting in Wataru, the 41st placer, being saved and continuing on to the second round. Masaki Uchida and Wataru Takahashi are revealed to be the candidates for 40th place, with Masaki Uchida taking 40th place. Masaya Kimura and Shogo Tajima are revealed to be the top 2 trainees, with Masaya Kimura being voted 1st with 1,476,946 votes. Anthonny Iinuma, Nalu Okubo, Takeru Goto, Hiromu Takatsuka, Yudai Sano, Renta Nishijima, Takumi Ozaki and Hiroto Nishi take 11th to 3rd places, respectively. The first round of elimination takes place. Trainees vote for their visual picks, with Hiroto Nishi, Renta Nishijima, and Hiroaki Morii voted as the top 3. The trainees reveal their nightly routine at their hotel rooms through vlogs. The events leading to the 1st elimination are shown. Hiromu Takatsuka takes 1st place and is further revealed to have taken first place overall as his team won the group battle.

The rehearsals and performances of the rest of the teams are shown. The teams practice and then perform the songs, with each individual being voted on separately by the studio audience, and with the members on the winning team for their song receiving a bonus of 3,000 votes. The options are " I Need U" by BTS, " AGEHA" by Generations from Exile Tribe, "&LOVE" by King & Prince, " Your Number" by SHINee, and " Infinity" by JO1. The first evaluation, the Group Battle, is announced, and they are organized into 10 teams of 6 members each 2 teams compete against each other on a pop song. The trainees vote for their center pick for the theme song from the new set of A-class trainees, with Masaya Kimura chosen to be the center. The boys are then given their new grades. Each video is then watched and evaluated by the mentors. The contestants film themselves performing the song individually. The trainees practice the choreography for the theme song, "Let Me Fly (~その未来へ~)", after which they will undergo a re-evaluation and have their grades reassigned. The trainees also perform their audition songs by team, and each trainee is graded individually from A to F, based on their overall talents, organizing them into temporary classes for training. Each trainee sits on the seat corresponding to their current rank, with Masaya Kimura taking 1st place. The 1st ranking announcement for the 60 trainees begins, with the studio having seats arranged in a pyramid numbered 1 to 60.

Auditions for the show took place from December 4 to for male Japanese residents aged 15–27 years old who were not tied to any talent agency. The group created from the show would be permanent and would be handled by Lapone Entertainment. In November 2020, Yoshimoto Kogyo and CJ E&M announced they would be co-producing a 2nd Japanese version of the Produce 101 reality competition show with the intention of debuting an 11-member boy band in 2021 for the global music market.
